Description
The BÜHLMANN MRP8/14 ELISA kit is designed for the quantitative determination of the heteropolymer of myeloidrelated protein MRP8/14 (Calprotectin, S100A8/S100A9) in serum. The test allows the selective measurement of MRP 8/14- antigen by sandwich ELISA. A monoclonal capture antibody (mAb) highly specific to the MRP8/14 heterodimeric and polymeric complexes respectively, is coated onto the microtiter plate. A second monoclonal detection antibody (Ab) conjugated to horseradish peroxidase (HRP) detects the MRP8/14 molecules bound to the monoclonal antibody coated onto the plate after a washing step. After incubation and a further washing step, tetramethylbenzidine (TMB) will be added (blue color formation) followed by a stopping reaction (change to yellow color). The absorption is measured at 450 nm